Correlation of Nitrite and Ammonia with Prevalence of Enterocytozoon hepatopenaei (EHP) in Shrimp (Litopenaeus vannamei) on Several Super-Intensive Ponds in East Java, Indonesia

AbstractEnterocytozoon hepatopenaei (EHP), a microsporidia parasite known to hinder shrimp growth by infecting its hepatopancreas, is recently an emerging infection for Litopenaeus vannamei farms in Indonesia. The present study was conducted investigate the relationship between nitrite and ammonia concentration with EHP super-intensive ponds. Shrimps water samples were collected from six ponds aquaculture stocking density of 5,000 shrimps each pond which located Pasuruan, Lamongan, Tuban Regency, sample taken laboratory measurement nitrite. detected spectrophotometer, PCR used detect 18S rRNA EHP. analysis that showed two positive L. vannamei, high nitrite, infected statistical significant correlation prevalence infections, where Pearson (r) 0.980 0.943, respectively. There increase four six. over 1mg/l could influence farms. Highlight ResearchAmmonia Enterocytozoon Super intensive ponds.The estimated 16.7%.PCR amplification ss-rRNA 510-bp obtained.